Glued DNA bridges and rainbow clusters

Chapters:

00:00-Flowcells

03:40-DNA glued to glass

09:00-Bridge amplification

15:50-Cluster density and patterned Flowcells

28:30-Illumina SBS summary

30:30-Limits

33:00-Multiplexing and Demultiplexing

- enseqlopedia on patterned vs unpatterned flowcell: http://enseqlopedia.com/2016/01/almost-everything-you-wanted-to-know-about-illumina-hiseq-4000-and-some-stuff-you-didnt/

- ClevaLabs on Illumina sequencing: https://www.youtube.com/watch?v=WKAUtJQ69n8

- Yourgenome on how the Illumina workflow works: https://www.yourgenome.org/facts/what-is-the-illumina-method-of-dna-sequencing/

- DNATech comparing Illumina Machines: https://dnatech.genomecenter.ucdavis.edu/illumina-high-throughput-sequencing/

- iBiology on sample preparation for Illumina sequencing: https://www.youtube.com/watch?v=PFwSe09dJX0

- StatQuest on how to use Illumina sequencing for RNA analysis: https://www.youtube.com/watch?v=tlf6wYJrwKY

- Illumina Flowcell and sequencing machines pictures: https://www.illumina.com/company/news-center/multimedia-images.html

- Nice explainer about paired-end sequencing: https://www.youtube.com/watch?v=gVrmfLXJRGM

Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany. Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ).

The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).

Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).

Clarification: Each Flowcell on the Illumina platform has 1-8 channels, each channel can be filled with multiple different samples - DR