Zipping rounds in zeptoliters

Chapters:

00:00 Intro

07:00 Laser

09:00 Zeptoliters

13:30 Polymerase speed

19:00 Error rate

22:00 SMRT Cell

25:45 ccs

30:00 Fragment size

34:30 Megaruptor

38:20 Data flow

47:00 SVs

53:00 MAS Seq

- Starter: https://www.nature.com/articles/s41366-021-00824-3

- Karobben on PacBio sequencing: https://karobben.github.io/2023/10/30/Bioinfor/PacBio/

- bioinformticamente explaining the technology: https://bioinformaticamente.com/2020/12/05/pacbio-sequencing/

- Basic Biochem explainer of SMRT sequencing: https://www.youtube.com/watch?v=H2gUmRf-K1c

- Genomics Lab explaining it: https://www.youtube.com/watch?v=vRhUSjhygDU

- PacBio explaining BAM Tags: https://pacbiofileformats.readthedocs.io/en/13.0/BAM.html

- Megaruptor3 explainer (how we make the right sized fragments) : https://www.youtube.com/watch?v=zVhqnkQL8zo

- How we select the right sized fragments: https://www.youtube.com/watch?v=A25C8xfBbq8

Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).

The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).

Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).