We have the same thing at home!

- yes, but in worse, cheaper or both

Qitan:

https://pmc.ncbi.nlm.nih.gov/articles/PMC8985760/

https://www.qitantech.com/

The QPinnacle2:

https://www.vcbeathealth.com/article/1821

CycloneSEQ, the MGI-internal clone:

https://en.cyclone-seq.com/nanopTech

https://www.prnewswire.com/news-releases/mgi-launches-new-nanopore-sequencing-products-with-advanced-cycloneseq-technology-302241816.html

https://www.biorxiv.org/content/10.1101/2024.08.19.608720v1

https://rrwick.github.io/2024/12/17/cycloneseq.html

https://gigabytejournal.com/articles/154

https://gigasciencejournal.com/blog/open-cycloneseq-benchmarking-for-complete-bacterial-genomes/

Polyseq:

https://polyseq.com/

Axbio:

https://www.axbio.cn/products/45.html

Geneus-tech:

http://www.geneus-tech.com/proddetail.aspx?id=1

Nice not up-to-date table of specs:

https://github.com/zhangtianyuan666/NanoporeReview2024

ONT to double promethion throughput: https://www.genomeweb.com/sequencing/jpm-oxford-nanopore-shares-plans-double-flow-cell-output-expand-menu-biopharma-market

Artificial Intelligence everywhere, and sometimes its useful.

Correction: apparently the Biostar Handbook is not free anymore :(

AlphaGenome:

https://omicsomics.blogspot.com/2026/02/non-coding-dnas-alpha-moment.html

https://www.alphagenomedocs.com/installation.html

https://www.genomeweb.com/informatics/google-alphagenome-users-scrutinize-ai-models-ability-predict-variant-effects

https://deepmind.google.com/science/alphagenome/

Bioinformatics Forums:

Bioinformatics mistakes on Biostars: https://www.biostars.org/p/7126/

Bioinformatics stackexchange:

https://bioinformatics.stackexchange.com/

Seqanswers:

https://www.seqanswers.com/forum/sequencing-technologies-companies

Vibecoding (current setup, top to bottom):

https://claude.ai/new

https://gemini.google.com/app

https://duck.ai/

If danger is something you enjoy, use this:

https://openclaw.ai/

YOU HAVE BEEN WARNED

Paper about old LLMs that can solve simple, old and solved bioinformatics problems:

https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1011511

Youtube has useful bioinformatics courses:

1: https://www.youtube.com/watch?v=3XFpxQF0J74&list=PLWVKUEZ25V95IKyBrxHtRTK_6Ig8Xi9-f

2: https://www.youtube.com/watch?v=LvtPXZHNoCc&list=PLeB-Dlq-v6taAXK6ZCGfqImrNWJzFt3p3

When do you get your Illumina sticker for your ONSO?

This is the first part of a two-parts news roundup.

PacBio News:

https://aseq.substack.com/p/pacbio-sells-short-read-tech-to-illumina

https://www.pacb.com/press_releases/pacbio-completes-sale-of-short-read-sequencing-assets/

https://albertvilella.substack.com/p/pacbio-sells-short-read-ip-to-illumina

The 6-base sequencing run:

https://www.pacb.com/wp-content/uploads/PAG-5hmC-Portik.pdf

https://www.nature.com/articles/s42003-025-07681-0

ONT's P2 solo drama:

https://omicsomics.blogspot.com/2026/01/p2-solo-ont-extends-support-but-not.html

For when matching and sticking is enough to get

Links:

Nice Youtube explainer: https://www.youtube.com/watch?v=NgRfc6atXQ8

Another nice one: https://www.youtube.com/watch?v=4bOY2Oh5tEQ

Explaining this and more: https://berkeleygenomics.org/articles/How_many_human_genomes_have_been_sequenced_.html

Since 2018, the West German Genome Center (⁠https://wggc.de/⁠) supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: ⁠https://ngs-kn.de/⁠) – network of NGS core facilities in Germany.

Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).

License Notice

This podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.

Attribution:

Podcast Title: Explain Podcast

Creators: Iuliia Novoselova, Daniel Rickert

Website: ⁠⁠⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠

After 454 now the second try by Roche: Axelios.

Preprint: https://www.biorxiv.org/content/10.1101/2025.02.19.639056v1

Read here about the PoC: https://www.pnas.org/doi/full/10.1073/pnas.1601782113

Current state (2025): https://www.nejm.org/doi/full/10.1056/NEJMc2512825

Correction: the 5B flow cell will arrive for the NovaseqX(+), not for the Nextseq2000

Also:

PacBio makes sequencing cheaper: https://www.pacb.com/press_releases/pacbio-announces-major-advances-for-revio-and-vega-to-lower-genome-cost-and-expand-multiomic-capabilities/⁠

Roche about SBX: https://sequencing.roche.com/global/en/article-listing/sequencing-platform-technologies.html

Roche from a conference: https://www.youtube.com/watch?v=YN2meswiCiQ

Since 2018, West German Genome Center (⁠https://wggc.de/⁠) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: ⁠https://ngs-kn.de/⁠) – network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).

License Notice

This podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.

Attribution:

Podcast Title: Explain Podcast

Creators: Iuliia Novoselova, Daniel Rickert

Website: ⁠⁠⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠

This time something different.

Sources:

https://www.23andme.com/privacy/

https://verbraucherschutzforum.berlin/2025-03-25/23andme-meldet-insolvenz-an-was-nutzer-jetzt-wissen-sollten-357738/

https://en.wikipedia.org/wiki/23andMe_data_leak

https://www.cshub.com/attacks/news/23andme-hacker-leaks-data

https://www.cbsnews.com/news/dna-privacy-at-home-tests-23andme-ancestrydna-sell-data-to-third-parties/

https://techcrunch.com/2025/03/24/23andme-faces-an-uncertain-future-so-does-your-genetic-data/

https://www.theverge.com/2024/9/13/24243986/23andme-settlement-dna-data-breach-lawsuit

https://newsroom.regeneron.com/news-releases/news-release-details/regeneron-enters-asset-purchase-agreement-acquire-23andmer-256/

The science of white blood cells, and much more

Chapters:

05:30 Immunology how?

22:05 22q11.2 deletion syndrome

34:30 Scientific collaboration

40:00 Data losses

50:00 Flip it!

53:00 outlook 2030

Links:

mini-immunology intro:

https://www.youtube.com/watch?v=k9QAyP3bYmc

22Q e. V. - zentraler Anlaufpunkt für Menschen mit Deletionssyndrom 22q11 oder Duplikationssyndrom 22q11 im deutschsprachigen Raum https://www.wirsind22q.de/aus-dem-verein/vorstand

Podcast for young immunologists from young immunologists https://theyoungimmunologist.buzzsprout.com/

Contact Nora here https://www.linkedin.com/in/nora-balzer-phd-253a767b

Some information about training courses and events https://www.immunology.org/traininghttps://dgfi.org/dgfi-en/academy-of-immunology/

Since 2018, West German Genome Center (⁠https://wggc.de/⁠) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: ⁠https://ngs-kn.de/⁠) – network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).

License Notice

This podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.

Attribution:

Podcast Title: Explain Podcast

Creators: Iuliia Novoselova, Daniel Rickert

Website: ⁠⁠⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠

Sharing a flowcell, reinvented

Chapters:

- 00:00 usegalaxy.org

- 07:55 Singular Genomics start

- 21:55 Max Reads

- 29:40 DNA Structure / ATAC-seq

Setup galaxy on local hardware:

https://galaxyproject.org/admin/get-galaxy/

G4 is competing with Illumina:

https://www.genengnews.com/topics/omics/fast-and-flexible-singulars-g4-takes-aim-at-illuminas-sequencing-superiority/

Singulars chemistry is one of many:

https://www.nature.com/articles/d41586-023-00512-4

How max reads works:

https://techwriting.singulargenomics.com/max-read/Max-Read-Kit-User-Guide-600026.pdf

Small intro to DNA Structures:

https://en.wikipedia.org/wiki/Nucleic_acid_quaternary_structure

Intro to ATAC-seq:

https://www.nature.com/articles/s41596-022-00692-9

PS:

As of 2025, Singular does not advertise DNA/RNA Sequencing on their G4 anymore, but shifted to spatial data, including their G4X: https://www.singulargenomics.com/

Since 2018, West German Genome Center (⁠https://wggc.de/⁠) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: ⁠https://ngs-kn.de/⁠) – network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).

License Notice

This podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.

Attribution:

Podcast Title: Explain Podcast

Creators: Iuliia Novoselova, Daniel Rickert

Website: ⁠⁠⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠