Pushing spaghetti through holes

Chapers:

00:00 Intro

11:15 Measuring Ions

17:00 Quality

23:00 Duplex

24:30 MinION

25:15 PromethION

28:20 Methylation

29:00 Direct RNA sequencing

38:25 Basecalling

46:00 Basecalling bias

53:50 Sequencing proteins

- Nanopore explainer: https://nanoporetech.com/applications/dna-nanopore-sequencing

- Oxsci explaining the technology: https://oxsci.org/pore-over-this-advances-in-dna-sequencing/

- History of Nanopore: https://www.whatisbiotechnology.org/index.php/science/summary/nanopore/nanopore-sequencing-makes-it-possible-to-decode-the

- Shmou’s Biology explaining Nanopore sequencing: https://www.youtube.com/watch?v=MlluAjhzXqI

- A direct comparison of Nanopore and Illumina output: https://medresearch.umich.edu/office-research/about-office-research/biomedical-research-core-facilities/advanced-genomics-core/technologies/next-generation-sequencing

- A nice Nanopore QC tool we use: https://hpc.nih.gov/apps/pycoQC.html

- How a MinION looks like: https://www.whatech.com/og/markets-research/medical/658327-global-nanopore-sequencing-market-2020

Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.

Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).

The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).

Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).