Glycosylation is a prevalent modification of proteins known to play diverse roles across a wide range of biological processes.

While glycoproteins are widely recognized as key molecular features involved in human health and disease, the analysis of protein glycosylation has historically been hampered by a lack of efficient and simple methods to characterize the analytically challenging glycan structures. Examples include structures exhibiting extensive heterogeneity and subtle isomeric features such as linkage and topological differences.

In this episode of Listen In, Dr. Thaysen-Andersen discusses opportunities and challenges in the burgeoning field of analytical glycoscience.

See the current landscape of glycobiology, learn about innovative analytical methods for N- and O-linked glycosylation profiling from biological specimens, and explore how advances in glycoanalytics enable new insights into human glycobiology.

MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.

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Liquid chromatography-mass spectrometry (LC-MS) is a crucial technique in many life sciences, including proteomics, metabolomics, pharmaceutical analysis, clinical diagnosis, and forensic science.

In this episode of Listen In, elevate your analysis and liquid chromatography. Learn what consumables, materials, and reagents can harm your LC-MS data for more accurate quantification using perfluoroalkyl substances (PFAS) as an example.

See how contamination from consumables, equipment, reagents, and non-specific binding of samples to syringe filters can make LC-MS quantification inaccurate.

Plus, explore the environmental impact of PFAS and how to choose syringe filters and other consumables for accurate quantification results.

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Holotomography has emerged as a helpful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation.

It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nuclei, nucleoli, mitochondria, and lipid droplets.

Furthermore, analysis of the measured individual cell data can elucidate the temporal 3D volumetric dynamics with the dry mass information.

This episode presents the latest development of a low-coherence holotomography imaging system, HT-X1, and its numerous applications to different types of biological specimens, ranging from unicellular organisms to multicellular specimens.

Plus, learn how to combine holotomography with downstream molecular analysis, such as cell biology, immunology, microbiology, material science, and in vitro diagnosis.

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Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/